Journal: Developmental cell

Article Title: Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization

doi: 10.1016/j.devcel.2017.12.023

Figure Lengend Snippet:

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Escherichia coli (BL21DE3) Agilent 200131 Escherichia Coli (XL10Gold) Agilent 200314 Chemicals, Peptides, and Recombinant Proteins SNAP-Cell TMR-Star NEB S9105S SNAP-Surface Alexa Fluor 647 NEB S9136S SNAP-Surface Alexa Fluor 488 NEB S9129S Dylight 405 NHS Ester ThermoFischer 46400 Alexa Fluor 647 NHS Ester ThermoFischer {"type":"entrez-nucleotide","attrs":{"text":"A37573","term_id":"2294435","term_text":"A37573"}} A37573 Biotinylated poly(L-lysine)-[g]-poly(ethylene-glycol) (PLL-PEG-Biotin SuSoS AG PLL(20)-G[3.5]-PEG(2)/PEG(3.4)-biotin(50%) Streptavidin ThermoFischer 21135 Trolox (6-hydroxy-2,5,6,7,8-tetramethylchroman-2-carbonsaure, 97%) Acros AC218940050 3,4-Dihydroxybenzoic Acid (Protocatechuic acid) Sigma-Aldrich 37580 Protocatachuate 3,4-Dioxygenase from Pseudomonas sp. Sigma-Aldrich P8279 κ-caesin from bovine milk Sigma-Aldrich C0406 Pierce Bovine Serum Albumin, Biotinylated Thermo-Fischer 209130 GpCpp (guanosine-5′-[(α, β) – methyleno]triphosphate, Sodium salt Jena Bioscience Nu-405 Paclitaxel Sigma-Aldrich T7402 Pluronic F-157 Sigma-Aldrich P2443 Glass cover slides (18×18-1.5) Fischer 12-541A Superfrost Microscope slides Fisher 12-550-143 Double Sided tape 3M 34-8517-0998-9 Adenosine 5′-triphosphate disodium salt hydrate Sigma-Aldrich A2383 Guanosine 5′-triphosphate sodium salt hydrate Sigma-Aldrich G8877 Bovine Serum Albumin Sigma-Aldrich A2058 Casein Sigma-Aldrich C7078 Methyl Cellulose Sigma-Aldrich M0387 Nonidet P 40 substitute (NP-40) Sigma-Aldrich 74385 PDMS (polydimethylsiloxane, sylgard 184) Sigma-Aldrich 761036 Dimethyl sulfoxide ACS Reagent Sigma-Aldrich 472301 Atto 488 NHS ester Sigma-Aldrich 41698 Recombinant DNA Plasmid: pET28-StrepII-SNAPf-Hook3 McKenney et al. Science 2014 Plasmid: pET28-StrepII-SNAPf-BicD2 McKenney et al. Science 2014 Software and Algorithms FIJI Schindelin et al. 2012 https://Fiji.sc/ GraphPad Prism GraphPad https://www.graphpad.com/scientific-software/prism/ μManager Edelstein et al. 2010 https://micro-manager.org/ Autocad 360 Autodesk https://client.autocad360.com/ Silhouette Studio Silhouette America https://www.silhouetteamerica.com/software Open in a separate window

Techniques: Virus, Recombinant, Microscopy, Plasmid Preparation, Software